A tris (2-carboxyethyl) phosphine (TCEP) related cleavage on cysteine-containing proteins

Reaction of Tris(2-carboxyethyl)phosphine (TCEP) with maleimide and alpha-haloacyl groups: anomalous elution of TCEP by gel filtration.

Tris-(2-carboxyethyl) phosphine significantly promotes the reaction of cisplatin with Sp1 zinc finger protein.

A widely used reducing agent tris-(2-carboxyethyl) phosphine significantly promotes the reaction of cisplatin with Sp1 zinc finger protein. This discovery clarifies the reactivity of cisplatin towards Sp1 zinc finger protein and implies that the reactions of platinum drugs could be largely influenced by small molecules with a strong trans effect.

Tris(2-methoxy­phen­yl)phosphine

In the title compound, C(21)H(21)O(3)P, the whole mol-ecule is disordered over two sets of positions with refined occupancies of 0.503 (1) and 0.497 (1). The dihedral angles between the three benzene rings are 72.9 (2)°, 82.9 (3)° and 70.0 (2)° in the major disorder component and the corresponding angles in the minor disorder component are 85.0 (2)°, 79.2 (2)° and 72.3 (2)°. The crystal structu...

A Comparison between the Sulfhydryl Reductants Tris(2-carboxyethyl)phosphine and Dithiothreitol for Use in Protein Biochemistry1

t t d The newly introduced sulfhydryl reductant tris(2arboxyethyl)phosphine (TCEP) is a potentially atractive alternative to commonly used dithiothreitol DTT). We compare properties of DTT and TCEP imortant in protein biochemistry, using the motor enyme myosin as an example protein. The reductants qually preserve myosin’s enzymatic activity, which is ensitive to sulfhydryl oxidation. When label...

Thiol-reactive molecule with dual-emission-enhancement property for specific prestaining of cysteine containing proteins in SDS-PAGE.

1-[4-(Bromomethyl)phenyl]-1,2,2-triphenylethene (2) was synthesized and evaluated for specific fluorescent prestaining of proteins containing cysteine (Cys) in SDS-PAGE. The molecule showed classic aggregation-induced emission (AIE) property in protein labeling and its quantum efficiency was further enhanced upon reacting with Cys. The parameters of reaction such as labeling time and concentrat...